Primer Design
Oligo 7
จัดทำโดย
นายณัฐพล ปลอดทอง
5336195 RACP/M
OLIGO Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes, and various kinds of probes including siRNA and molecular beacons.
Based on the most up-to date nearest neighbor thermodynamic data, Oligo's search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers.
Oligo 7 can automatically select multiplex primers, process sequence files in batch modes, automatically design PCR primers to cover multiple DNA regions in just one search and automatically find primer/probe sets for real time PCR or finds nested primers sets. Oligo search protocols (scoring system) may be customized in detail, so you may optimize the results according to your specific needs.
OLIGO Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes, and various kinds of probes including siRNA and molecular beacons.
Based on the most up-to date nearest neighbor thermodynamic data, Oligo's search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers.
Oligo 7 can automatically select multiplex primers, process sequence files in batch modes, automatically design PCR primers to cover multiple DNA regions in just one search and automatically find primer/probe sets for real time PCR or finds nested primers sets. Oligo search protocols (scoring system) may be customized in detail, so you may optimize the results according to your specific needs.
1. File > Reset > Original Defaults
2.Change > Current Oligo Length > Add primer Length (such as 20) > OK
5. Show number of Pairs Accepted
6. Upper and Lower Primers
